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Improved biocompatibility of novel poly(L-lactic acid)/β-tricalcium phosphate scaffolds prepared by an organic solvent-free method

机译:通过无溶剂方法制备的新型聚(L-乳酸)/β-磷酸三钙支架的生物相容性得到改善

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摘要

A porous poly(L-lactic acid)/β-tricalcium phosphate (PLLA/β-TCP) composite scaffold was fabricated using a novel technique comprising powder mixing, compression molding, low-temperature treatment, and particulate leaching without any organic solvent. The effect of this scaffold on osteoblast proliferation and differentiation was evaluated in vitro. The fabricated scaffold had a homogeneously interconnected porous structure with a porosity of 70% and compressive strength of 1.35 MPa. The methylthiazol tetrazolium values and alkaline phosphatase (ALP) activity of osteoblasts seeded on the solvent-free scaffold were significant higher than those of the control. Using real-time PCR, gene expressions of ALP, osteocalcin, and type 1 collagen were shown to be upregulated. As the method does not use any organic solvent, it eliminates problems associated with organic solvent residue and therefore improves the cell compatibility. It has a promising potential for the preparation of porous scaffold for bone tissue engineering.
机译:多孔聚(L-乳酸)/β-磷酸三钙(PLLA /β-TCP)复合支架是使用一种新颖的技术制造的,该技术包括粉末混合,压缩成型,低温处理和无任何有机溶剂的颗粒浸出。在体外评估了该支架对成骨细胞增殖和分化的作用。制成的支架具有均匀互连的多孔结构,其孔隙率为70%,抗压强度为1.35 MPa。在无溶剂支架上接种的成骨细胞的甲基噻唑四唑鎓值和碱性磷酸酶(ALP)活性均显着高于对照。使用实时PCR,显示ALP,骨钙素和1型胶原蛋白的基因表达上调。由于该方法不使用任何有机溶剂,因此消除了与有机溶剂残留物相关的问题,因此提高了电池兼容性。它具有制备用于骨组织工程的多孔支架的潜力。

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